产品货号：
KD1598
中文名称：
His-Tag mouse monoclonal antibody
英文名称：
His-Tag mouse monoclonal antibody
产品规格：
100μL
发货周期：
1～3天
产品价格：
询价
保存：-20℃
Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds
of immunoassays,helping researchers identify,detect,and purify polyhistidine fusion proteins in bacteria,insect
cells,and mammalian cells.His-tag mouse mAb recognizes His-tag placed at N-terminal,C-terminal,and internal
regions of fusion proteins.
Product Name:His-tag mouse monoclonal antibody
Clone Number:6E8
Isotype:IgG1
Immunogen:A synthetic peptide HHHHHH coupled to KLH.
Tested applications and dilutions:WB 1:5000～2000 IP 1:200 IF 1:1000
Produce anti-HIS antibody affinity column purify HIS recombinant proteins
Form:Liquid
Concentration:1mg/ml in PBS,pH7.4,containing 0.02% sodium azide and 50% glycerol
Storage:His antibody is stable for 1 month at 2～8℃.For long term storage,Aliquot and store at -20℃ or below.
Avoid repeated freezing and thawing cycles.
A:HEK 293 cell lysate without transfected
B:HEK 293 cell lysate transfected with his-tag protein
C:IP (PBS + anti-HIS mAb 4℃ overnight)
D:IP (HEK293 transfected+protein G+anti-HIS mAb 4℃ overnight))
E:IP (HEK293 transfected+RIPA+Protein G 4℃ overnight)
F:Multi Tag Recombinant protein (expressed in E.coli)
TP=Target Protein HC=Heavy chain LC=Light Chain
IF analysis of 293T cells transfected with a HIS-tagged protein,
using EASYbio HIS-Tag (6E8) Mouse mAb at a 1:2000 dilution
(blue DAPI,red anti-HIS)
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